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          基因商城首頁 > 試劑 > 分子生物學 > CRISPR/Cas9基因編輯 > 突變

          EnGen? 基因編輯突變檢測試劑盒

          ?EnGenTM??Mutation Detection Kit

          品牌:NEB 貨號:E3321S 注冊及訂單:注冊及訂單 咨詢QQ 產品及技術:產品及技術 咨詢QQ

          商城價:

          ¥2116.00
          目錄價:¥2489.00

          特價促銷:

          ¥2116.00
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          庫存貨期:
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          • 基因生物技術國際貿易(上海)有限公司 發貨并提供售后服務。
            本產品僅限科研用途。

          產地:USA
          存儲條件:-20
          EnGen??基因編輯突變檢測試劑盒 ???, EnGen? Mutation Detection Kit, 25 rxns;The EnGen Mutation Detection Kit provides reagents for detection of on-target genome editing events. In the first step, targeted regions from cells whose genomes were targeted (i.e. CRISPR/Cas9, TALENs, Zinc-finger Nucleases) are amplified using Q5 Hot Start High-Fidelity 2X Master Mix. Upon denaturation and re-annealing, heteroduplexes are formed when mutations from insertions and deletions (indels) are present in the amplicon pool. In the second step, annealed PCR products are digested with EnGen T7 Endonuclease I, a structure-specific enzyme that will recognize mismatches larger than 1 base. Both strands of the DNA are cut when a mismatch is present, which results in the formation of smaller fragments. Analysis of the resulting fragments provides an estimate of the efficiency of the genome editing experiments. The EnGen Mutation Detection Kit includes a Control Template and Primer Mix that can be used as a control for the PCR reaction and T7 Endonuclease I digestion. The Control Template and Primer Mix provided contains two plasmids and primers that when amplified, denatured and re-annealed will form heteroduplexes that contain a 10-base insertion. This structure is a substrate for T7 Endonuclease I. The digestion of the 600 bp heteroduplex containing amplicon yields products of 200 bp and 400 bp. 600 bp parental homoduplexes are uncleaved, and are easily distinguished from cleaved heteroduplexes when separated and visualized by agarose gel electrophoresis or fragment analysis instrument. The protocol has been optimized so that PCR products generated by the Q5 Hot Start High-Fidelity 2X Master Mix can be introduced directly into the T7 Endonuclease I digestion without the need for purification. Digestion of the heteroduplex is complete in only 15 minutes, and Proteinase K is included to stop the reaction efficiently. Additional Q5 Hot Start High-Fidelity 2X Master Mix is also included to allow for optimization of target site amplification before digestion.
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          • 咨詢標題:[咨詢產品]NEB / E3321S / ?EnGenTM??Mutation Detection Kit
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